光合毒理学分析揭示花椒叶浸提液对莱茵衣藻的化感效应机制
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https://doi.org/10.70693/cjst.v1i1.662关键词:
花椒叶浸提液；非靶向代谢组；叶绿素荧光；抑藻剂；莱茵衣藻摘要
摘要: 花椒（Zanthoxylum bungeanum）作为我国传统药食同源植物，其果实因富含挥发油、生物碱及黄酮类成分而广泛应用于食品与医药领域，但花椒叶作为采收副产物长期面临资源浪费与环境污染问题。为挖掘花椒叶潜在应用价值，本研究聚焦花椒叶次级代谢产物组成及其对水生初级生产者莱茵衣藻（Chlamydomonas reinhardtii）的化感效应。通过非靶向代谢组学技术分析花椒叶浸提液化学成分，发现其主要含酚酸类与黄酮类化合物，其中黄酮类化合物组分占比显著。结合急性毒理实验与叶绿素荧光技术，发现添加0.5 mg/L与1.0 mg/L花椒叶浸提液的藻细胞Fv/Fm值分别较对照组降低了8.32%和60.50%，表明不同剂量浸提液对莱茵衣藻光合功能的浓度依赖性抑制效应。进一步机制研究表明，花椒叶浸提液处理破坏了藻株放氧复合体的结构，影响了能量的捕获和分配，抑制了光合电子的传递效率。本研究为化感抑藻剂的开发提供理论依据与数据支撑，并在水体生态修复方面具有广阔的应用前景。
基金项目: 聊城市重点研发计划（2024YD01），聊城大学大学生创新创业训练计划项目（CXCY347）。
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